Bio-molecular Analysis Platform
Please note that the service is no longer in place. However, if you want to use these techniques then you can contact us on a collaborative basis.
Contact: Prof. Teresa Fitzpatrick
The Bio-molecular Analysis (BMA) platform is one of three technology platforms of the Swiss Plant Science Web (SPSW). It is the technology platform of the Arc Lémanique Plant Science (ALPS) and provides the appropriate expertise and techniques to obtain information about the functionalities of individual bio-molecules. The BMA services are split between the Universities of Lausanne and Geneva. Fourier-Transform IR Spectroscopy and Microscopy is offered in Lausanne. Protein analytical services are provided at the Department of Botany and Plant Biology of the University of Geneva.
This page briefly presents the services provided by the BMA platform in Geneva. For further information see the description of the platform on the website of the ALPS.
Services of the BMA platform Geneva
The BMA Platform Geneva provides state of the art protein analytical services. After a period of genomics and proteomics, the emphasis on the characterization of particular biochemical systems is reinforced. Our platform offers technology to analyse protein systems, their interaction and complexes. Currently, the Geneva Bio-molecular Analysis Platform offers 3 major services:
- Multi Angle Light Scattering (MALS) analysis
MALS is used to determine the oligomeric state, the molecular mass Mw and the quality of a biological macromolecule or macromolecular complex. It is coupled online to size exclusion chromatography. MALS analysis is performed with a miniDAWN TREOS instrument from Wyatt Technology.
- Isothermal Titration Calorimetry (ITC)
ITC is a non-invasive technique to determine precise binding parameters (enthalpy, entropy, and binding constants) for molecular interactions between proteins, nucleic acid and chemicals. It is also a powerful method to analyse enzyme kinetics. ITC analysis is performed with an iTC200 instrument from Microcal/GE Healthcare.
- Consulting in recombinant protein technology (expression & purification)
We will assist researchers of the Swiss Plant Science Web to prepare high quality protein samples for biophysical analysis by MALS, ITC, and other state of the art methods.
BMA Geneva teaches biophysical methods and concepts in recombinant protein technology to users of the platform and students of the Swiss Plant Science Web. We are offering seminars about the BMA platform to member departments of the Swiss Plant Science Web. In 2010 the BMA visited 8 participating SPSW universities to introduce the services and technologies of the new BMA Platform.
- Biophysical methods (ITC, MALS) to study protein – ligand interactions
Date: April 25 - 27, 2012 (3 days)
Location: University of Geneva, Science III, Room 0035
Colinas M., Shaw H.V., Loubery S., Kaufmann M., Moulin M. & Fitzpatrick T.B.: A pathway for repair of NAD(P)H in plants.
J Biol Chem 2014. PMID: 24706747.
Moccand C., Boycheva S., Surriabre P., Tambasco-Studart M., Raschke M., Kaufmann M. & Fitzpatrick T.B.: The Pseudoenzyme PDX1.2 Boosts Vitamin B6 Biosynthesis under Heat and Oxidative Stress in Arabidopsis.
J Biol Chem 2014, 289(12):8203-16. PMID: 24505140.
Moccand C., Kaufmann M. & Fitzpatrick T.B.: It takes two to tango: defining an essential second active site in pyridoxal 5'-phosphate synthase.
PLoS One 2011, 6(1):e16042. PMID: 21283685.
Publications acknowledging analytics performed at the BMA platform
Huet T., Miannay F.A., Patton J.R. & Thore S.: Steroid receptor RNA activator (SRA) modification by the human pseudouridine synthase 1 (hPus1p): RNA binding, activity, and atomic model.
PLoS One 2014, 9(4):e94610. PMID: 24722331.
Bissig C., Lenoir M., Velluz M.C., Kufareva I., Abagyan R., Overduin M. & Gruenberg J.: Viral infection controlled by a calcium-dependent lipid-binding module in ALIX.
Dev Cell 2013, 25(4):364-73. PMID: 23664863.
Coquille S., Roux C., Mehta A., Begley T.P., Fitzpatrick T.B. & Thore S.: High-resolution crystal structure of the eukaryotic HMP-P synthase (THIC) from Arabidopsis thaliana.
J Struct Biol 2013, 184(3):438-44. PMID: 24161603.
Coquille S., Roux C., Fitzpatrick T.B. & Thore S.: The last piece in the vitamin B1 biosynthesis puzzle: structural and functional insight into yeast 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase.
J Biol Chem 2012, 287(50):42333-43. PMID: 23048037.
Kaufmann M.: SPSW Technology Platforms — Close Up: Technology Platform Geneva.
Swiss Plant Science Web Newsletter, 2011/1. Link.
Please do acknowledge the platform with a sentence such as:
Data analyzed in this publication were generated at the Bio-molecular Analysis Platform Geneva (http://biveg.unige.ch/bma).
Also do not forget to send us the references of your new publications!